Lymphatic Endothelial Tumors Induced by Intraperitoneal Injection of Incomplete Freund's Adjuvant
Identifieur interne : 00AD97 ( Main/Exploration ); précédent : 00AD96; suivant : 00AD98Lymphatic Endothelial Tumors Induced by Intraperitoneal Injection of Incomplete Freund's Adjuvant
Auteurs : Sabrina Mancardi [Italie] ; Giorgio Stanta [Italie] ; Nelson Dusetti [Italie] ; Marco Bestagno [Italie] ; Lotta Jussila [Finlande] ; Marina Zweyer [Italie] ; Giancarlo Lunazzi [Italie] ; Daniel Dumont [Canada] ; Kari Alitalo [Finlande] ; Oscar R. Burrone [Italie]Source :
- Experimental Cell Research [ 0014-4827 ] ; 1999.
English descriptors
Abstract
Endothelial cells form the inner lining of blood and lymphatic vessels. In mice, only tumors of the blood vessel endothelium (haemangiomas) have been thus far reported. Here we describe a highly reproducible method for the induction of benign tumors of the lymphatic endothelial cells (lymphangiomas) in mice by intraperitoneal injection of incomplete Freund's adjuvant. Morphological and histopathological studies of the lesions revealed the presence of cells at various levels of vascular development. The lymphangiomas developed in the peritoneal cavity and expressed the endothelial markers CD31/PECAM (platelet endothelial cell adhesion molecule), CD54/ICAM-1 (InterCellular Adhesion Molecule-1), and CD102/ICAM-2, as well as the vascular endothelial growth factor (VEGF) receptor Flk-1, the endothelial cell specific receptors Tie-1 and Tie-2 and the lymphatic endothelial cell specific Flt4 receptor as shown byin situhybridization. The Flk-1 and Flt4 receptors were also identified in immunoblots of the tumors and in cells cultured from them. When induced in β-galactosidase knock-in Flt4+/−mice, the tumor endothelia could be stained blue in a number of tumor cells although the staining was of lower intensity than in normal lymphatic vessels. The tumor-derived cells could be propagatedin vitroand they spontaneously differentiated, forming vessel-like structures. Murine lymphangiomas thus represent a highly reproducible and convenient source of lymphatic endothelial cells.
Url:
DOI: 10.1006/excr.1998.4270
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001D91
- to stream Istex, to step Curation: 001D91
- to stream Istex, to step Checkpoint: 003717
- to stream Main, to step Merge: 00B415
- to stream Main, to step Curation: 00AD97
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lymphatic Endothelial Tumors Induced by Intraperitoneal Injection of Incomplete Freund's Adjuvant</title><author><name sortKey="Mancardi, Sabrina" sort="Mancardi, Sabrina" uniqKey="Mancardi S" first="Sabrina" last="Mancardi">Sabrina Mancardi</name></author><author><name sortKey="Stanta, Giorgio" sort="Stanta, Giorgio" uniqKey="Stanta G" first="Giorgio" last="Stanta">Giorgio Stanta</name></author><author><name sortKey="Dusetti, Nelson" sort="Dusetti, Nelson" uniqKey="Dusetti N" first="Nelson" last="Dusetti">Nelson Dusetti</name></author><author><name sortKey="Bestagno, Marco" sort="Bestagno, Marco" uniqKey="Bestagno M" first="Marco" last="Bestagno">Marco Bestagno</name></author><author><name sortKey="Jussila, Lotta" sort="Jussila, Lotta" uniqKey="Jussila L" first="Lotta" last="Jussila">Lotta Jussila</name></author><author><name sortKey="Zweyer, Marina" sort="Zweyer, Marina" uniqKey="Zweyer M" first="Marina" last="Zweyer">Marina Zweyer</name></author><author><name sortKey="Lunazzi, Giancarlo" sort="Lunazzi, Giancarlo" uniqKey="Lunazzi G" first="Giancarlo" last="Lunazzi">Giancarlo Lunazzi</name></author><author><name sortKey="Dumont, Daniel" sort="Dumont, Daniel" uniqKey="Dumont D" first="Daniel" last="Dumont">Daniel Dumont</name></author><author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name><affiliation><country>Finlande</country><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName><orgName type="university">Université d'Helsinki</orgName></affiliation></author><author><name sortKey="Burrone, Oscar R" sort="Burrone, Oscar R" uniqKey="Burrone O" first="Oscar R." last="Burrone">Oscar R. Burrone</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:415476C1DCA89A1BF35E4E3C4BCB3266C6DE5997</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1006/excr.1998.4270</idno><idno type="url">https://api.istex.fr/document/415476C1DCA89A1BF35E4E3C4BCB3266C6DE5997/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001D91</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D91</idno><idno type="wicri:Area/Istex/Curation">001D91</idno><idno type="wicri:Area/Istex/Checkpoint">003717</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">003717</idno><idno type="wicri:doubleKey">0014-4827:1999:Mancardi S:lymphatic:endothelial:tumors</idno><idno type="wicri:Area/Main/Merge">00B415</idno><idno type="wicri:Area/Main/Curation">00AD97</idno><idno type="wicri:Area/Main/Exploration">00AD97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Lymphatic Endothelial Tumors Induced by Intraperitoneal Injection of Incomplete Freund's Adjuvant</title><author><name sortKey="Mancardi, Sabrina" sort="Mancardi, Sabrina" uniqKey="Mancardi S" first="Sabrina" last="Mancardi">Sabrina Mancardi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Padriciano 99, 34012, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Stanta, Giorgio" sort="Stanta, Giorgio" uniqKey="Stanta G" first="Giorgio" last="Stanta">Giorgio Stanta</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Anatomia Patologica, Universitá degli Studi di Trieste, 34100, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Dusetti, Nelson" sort="Dusetti, Nelson" uniqKey="Dusetti N" first="Nelson" last="Dusetti">Nelson Dusetti</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Padriciano 99, 34012, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Bestagno, Marco" sort="Bestagno, Marco" uniqKey="Bestagno M" first="Marco" last="Bestagno">Marco Bestagno</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Padriciano 99, 34012, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Jussila, Lotta" sort="Jussila, Lotta" uniqKey="Jussila L" first="Lotta" last="Jussila">Lotta Jussila</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>Molecular/Cancer Biology Laboratory, Haartman Institute, University of Helsinki, P.O.B. 21 (Haartmaninkatu 3), Sf-00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Zweyer, Marina" sort="Zweyer, Marina" uniqKey="Zweyer M" first="Marina" last="Zweyer">Marina Zweyer</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Morfologia Umana Normale, Universitá degli Studi di Trieste, 34100, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Lunazzi, Giancarlo" sort="Lunazzi, Giancarlo" uniqKey="Lunazzi G" first="Giancarlo" last="Lunazzi">Giancarlo Lunazzi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Padriciano 99, 34012, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Dumont, Daniel" sort="Dumont, Daniel" uniqKey="Dumont D" first="Daniel" last="Dumont">Daniel Dumont</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Biophysics, University of Toronto and Amgen and Ontario Cancer Institutes, Toronto, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>Molecular/Cancer Biology Laboratory, Haartman Institute, University of Helsinki, P.O.B. 21 (Haartmaninkatu 3), Sf-00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName><orgName type="university">Université d'Helsinki</orgName></affiliation></author><author><name sortKey="Burrone, Oscar R" sort="Burrone, Oscar R" uniqKey="Burrone O" first="Oscar R." last="Burrone">Oscar R. Burrone</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Padriciano 99, 34012, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Experimental Cell Research</title><title level="j" type="abbrev">YEXCR</title><idno type="ISSN">0014-4827</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">246</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="368">368</biblScope><biblScope unit="page" to="375">375</biblScope></imprint><idno type="ISSN">0014-4827</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-4827</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Flt4</term><term>Freund's adjuvant</term><term>lymphangioma</term><term>lymphatic endothelium</term><term>tyrosine kinase receptors</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Endothelial cells form the inner lining of blood and lymphatic vessels. In mice, only tumors of the blood vessel endothelium (haemangiomas) have been thus far reported. Here we describe a highly reproducible method for the induction of benign tumors of the lymphatic endothelial cells (lymphangiomas) in mice by intraperitoneal injection of incomplete Freund's adjuvant. Morphological and histopathological studies of the lesions revealed the presence of cells at various levels of vascular development. The lymphangiomas developed in the peritoneal cavity and expressed the endothelial markers CD31/PECAM (platelet endothelial cell adhesion molecule), CD54/ICAM-1 (InterCellular Adhesion Molecule-1), and CD102/ICAM-2, as well as the vascular endothelial growth factor (VEGF) receptor Flk-1, the endothelial cell specific receptors Tie-1 and Tie-2 and the lymphatic endothelial cell specific Flt4 receptor as shown byin situhybridization. The Flk-1 and Flt4 receptors were also identified in immunoblots of the tumors and in cells cultured from them. When induced in β-galactosidase knock-in Flt4+/−mice, the tumor endothelia could be stained blue in a number of tumor cells although the staining was of lower intensity than in normal lymphatic vessels. The tumor-derived cells could be propagatedin vitroand they spontaneously differentiated, forming vessel-like structures. Murine lymphangiomas thus represent a highly reproducible and convenient source of lymphatic endothelial cells.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Finlande</li><li>Italie</li></country><region><li>Uusimaa</li></region><settlement><li>Helsinki</li></settlement><orgName><li>Université d'Helsinki</li></orgName></list><tree><country name="Italie"><noRegion><name sortKey="Mancardi, Sabrina" sort="Mancardi, Sabrina" uniqKey="Mancardi S" first="Sabrina" last="Mancardi">Sabrina Mancardi</name></noRegion><name sortKey="Bestagno, Marco" sort="Bestagno, Marco" uniqKey="Bestagno M" first="Marco" last="Bestagno">Marco Bestagno</name><name sortKey="Burrone, Oscar R" sort="Burrone, Oscar R" uniqKey="Burrone O" first="Oscar R." last="Burrone">Oscar R. Burrone</name><name sortKey="Dusetti, Nelson" sort="Dusetti, Nelson" uniqKey="Dusetti N" first="Nelson" last="Dusetti">Nelson Dusetti</name><name sortKey="Lunazzi, Giancarlo" sort="Lunazzi, Giancarlo" uniqKey="Lunazzi G" first="Giancarlo" last="Lunazzi">Giancarlo Lunazzi</name><name sortKey="Stanta, Giorgio" sort="Stanta, Giorgio" uniqKey="Stanta G" first="Giorgio" last="Stanta">Giorgio Stanta</name><name sortKey="Zweyer, Marina" sort="Zweyer, Marina" uniqKey="Zweyer M" first="Marina" last="Zweyer">Marina Zweyer</name></country><country name="Finlande"><region name="Uusimaa"><name sortKey="Jussila, Lotta" sort="Jussila, Lotta" uniqKey="Jussila L" first="Lotta" last="Jussila">Lotta Jussila</name></region><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name></country><country name="Canada"><noRegion><name sortKey="Dumont, Daniel" sort="Dumont, Daniel" uniqKey="Dumont D" first="Daniel" last="Dumont">Daniel Dumont</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00AD97 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 00AD97 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:415476C1DCA89A1BF35E4E3C4BCB3266C6DE5997
|texte= Lymphatic Endothelial Tumors Induced by Intraperitoneal Injection of Incomplete Freund's Adjuvant
}}
| This area was generated with Dilib version V0.6.31. |  |